On Friday, we have microbiology class with Dr. Wan in afternoon. Afternoon usually hot and I was really sleepy before I enter the class. π΄π΄π΄But once Dr. Wan enter our class, it will make me fresh because Dr. Wan make some fun and the way Dr teach us are quiet different from others. On Friday, I learned about the microscopy. π¬π¬π¬ The history of the microscopes
Microscopy is the technical field of using microscopes to view objects and areas of objects that cannot be seen with the naked eye ( objects that are not within the resolution range of the normal eye). There are three well-known branches of microscopy which are optical, electron and scanning probe microscopy. Goals of microscopes are to produce a magnified image of the specimen, separate the details in the image, and render the details visible to the human eye or camera. Microscopy have two categories which are light microscopes and electron microscope. In light microscope have many varieties which are bright-field microscopes, dark-field microscopes, phase-contrast microscope, fluorescence microscopes and confocal microscope. I got learned about the parts of microscope which are ocular, body, nosepiece, objective lens, arm, mechanical stage, substage condenser, diaphragm, base with light source, field diaphragm lever, light intensity control, coarse focus adjustment knob, fine focus adjustment knob and the stage adjustment knobs. Bright-field microscope that use in lab requires stain and produce a dark image against a brighter background . Dark-field microscope image is formed by light reflected or refracted by specimen and produces a bright image of the object against a dark background. The phase-contrast microscope converts differences in refractive index into detected variations in light intensity. The phase-contrast is excellent way to observe living cells and stain is not necessary view internal structures of living organisms. The fluorescence microscopes exposes specimen to ultraviolet or blue light and the specimens usually stained with fluorochromes. Cell contains natural fluorescent substances example (chlorophyll) has been treated with fluorescent dye. The confocal microscopy is confocal scanning laser microscopy creates sharp, composite 3D image of specimens by using laser beam, aperture to eliminate stray light and computer interface. Confocal microscopy is the numerous applications including study of biofilms.
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